The Pasteur Museum is housed in the apartment where Louis Pasteur spent his final seven years and offers a rare behind-the-scenes look at the living and working environment of the world-renowned scientist. Visitors can gain a unique insight into his everyday life alongside his wife and can admire his rich and diverse scientific work.
The Institut Pasteur’s scientific strategy focuses on developing original and innovative topics and promoting interdisciplinary and multidisciplinary cooperation and approaches. The Institut Pasteur teams have access to the technological resources needed to speed up and further improve the quality of their outstanding research.
Ever since the introduction of the world’s first "Technical Microbiology" course in 1889, teaching has been a priority for the Institut Pasteur. The Institut Pasteur has an international reputation for quality teaching that attracts students from all over the world who come to further their training or top up their degree programs.
The mission of the Industrial Partnership team is to detect, promote, assist and protect the inventive activities from research (inventions, know-how and biological materials) conducted at the Institut Pasteur (and in some Institutes of its international network), and transfer there to industrial and/or institutional partners, in order to serve the patient needs and for the benefit of the society, as well as to contribute to sustainability of the Institut Pasteur’s resources.
With international courses, PhD and postdoctoral traineeship, each institute of the Institut Pasteur International Network (RIIP) contributes to the transmission of knowledge with the training of young researchers all around the world. In this context, doctoral and postdoctoral programmes, study and traineeship fellowships are available to scientists. Alongside training, dynamism and attractiveness of RIIP will result in the creation of 4-year group for the young researchers.
Cell Biology of the Infection by Listeria monocytogenes
[Pizarro-Cerda-Cerda et al., Cold Spring Harbor Perspectives in Medecine 2012]
Intracellular cell cycle of L. monocytogenes. L. monocytogenes binds to epithelial host cells and promotes its own uptake in a process mediated by the two bacterial surface proteins InlA and InlB. The secreted poreforming toxin LLO (together with the bacterial phospholipases PlcA and PlcB, depending on the cell type) promotes vacuolar rupture and bacterial escape to the cytoplasm, where L. monocytogenes can replicate efficiently. Surface expression of ActA allows intracellular bacteria to polymerize host cell actin and to generate actin comet tails that propel L. monocytogenes through the cytoplasm and through membrane protrusions into neighboring cells. There, bacteria localize in a double membrane vacuole, which can be lysed by LLO, PlcA, and PlcB to start a new infection cycle.
[Cossart, PNAS 2011]
The infection by L. monocytogenes in vitro. Schematic representation of the roles played by several virulence factors.
[Cossart, PNAS 2011]
The infection by L. monocytogenes in vitro. The steps of the infection are schematically shown together with the bacterial factors involved and the corresponding EM images.
[Hamon et al., Nature Reviews Microbiology 2006]
Schematic representation and electron micrographs of the Listeria monocytogenes life cycle. a) L. monocytogenes induces its entry into a non-professional phagocyte. b) Bacteria are internalized in a vacuole (also known as a phagosome). c,d) The membrane of the vacuole is disrupted by the secretion of two phospholipases, PlcA and PlcB, and the pore-forming toxin listeriolysin O. Bacteria are released into the cytoplasm, where they multiply and start to polymerize actin, as observed by the presence of the characteristic actin tails. e) Actin polymerization allows bacteria to pass into a neighbouring cell by forming protrusions in the plasma membrane. f) On entry into the neighbouring cell, bacteria are present in a double-membraned vacuole, from which they can escape to perpetuate the cycle. F-actin, filamentous actin.
[Pizarro-Cerda-Cerda et al., The Journal of Pathology 2006]
The intracellular cycle of L. monocytogenes. L. monocytogenesis able to induce its entry into target cells mainly by the activity of two invasion proteins, InlA and InlB (1). The bacteria are initially trapped within a phagocytic vacuole (2), but through the activity of the hAemolysin LLO and two other phospholipases the parasite-containing compartment is lysed. (3). Once in the cytoplasm, the freed bacteria are able to multiply and also to polymerize cellular actin (4). L. monocytogenes moves inside the cytosol of infected cells thanks to the actin-based motility system, until it encounters the plasma membrane of the infected cell: the bacteria then push this membrane and create an invagination in the membrane of a neighbouring cell, invading it (5). The bacteria are then found in a double membrane-bound compartment (6): these membranes are lysed again due to the activity of LLO and the phospholipases (7), and L. monocytogenesis ready to start a new infection cycle.
[Kocks et al., Cell 1992]
Thin Sections of lntracytoplasmic Wild-Type, Mutant LUTlP, and p/cB Mutant L. monocytogenes 3.5 hr Postinfection
J774 macrophages were infected for 30 min with 1.5 bacteria per cell and incubated in medium containing gentamicin.
(a) Wild-type bacterium surrounded by a dense layer of actin filaments.
(b) Wild-type bacterium associated with a long comet tail of actin.
(c and d) LUTlP mutant bacteria were not associated with microfilaments. Only some loose granular material seems to stick to the surface.
(e) LUTlP mutant bacteria multiplying freely in the cytoplasm.
(f and g) p/cB mutant bacteria associated with F-actin, similar to wild-type bacteria.
Bars represent 0.5 pm.
[Tilney et al., The Journal of Cell Biology 1989]
Stages in the entry, growth, movement, and spread of Listeria from one macrophage to another. Photographs illustrating all these intermediate stages have been presented in the figures.
Updated on 13/05/2014
Unité Interactions Bactéries-Cellules
INSERM U604 INRA USC2020
25, Rue du Docteur Roux
75724 Paris Cedex 15 FRANCE
Phone: + 33 (1) 45 68 88 41
Secretary: + 33 (1) 40 61 30 32
Fax: + 33 (1) 45 68 87 06
Our laboratory is located on the ground floor at the 53C entrance of the Roux Building (25, rue du Docteur Roux)
The metro stations Pasteur (line 6) and Volontaires (line 12) are within a 5 min walking distance from the Pasteur Institute.
The bus stop Pasteur (bus 95, towards Porte de Vanves) is located next to the Pasteur Institute main entrance.