The Pasteur Museum is housed in the apartment where Louis Pasteur spent his final seven years and offers a rare behind-the-scenes look at the living and working environment of the world-renowned scientist. Visitors can gain a unique insight into his everyday life alongside his wife and can admire his rich and diverse scientific work.
The Institut Pasteur’s scientific strategy focuses on developing original and innovative topics and promoting interdisciplinary and multidisciplinary cooperation and approaches. The Institut Pasteur teams have access to the technological resources needed to speed up and further improve the quality of their outstanding research.
Ever since the introduction of the world’s first "Technical Microbiology" course in 1889, teaching has been a priority for the Institut Pasteur. The Institut Pasteur has an international reputation for quality teaching that attracts students from all over the world who come to further their training or top up their degree programs.
The mission of the Industrial Partnership team is to detect, promote, assist and protect the inventive activities from research (inventions, know-how and biological materials) conducted at the Institut Pasteur (and in some Institutes of its international network), and transfer there to industrial and/or institutional partners, in order to serve the patient needs and for the benefit of the society, as well as to contribute to sustainability of the Institut Pasteur’s resources.
With international courses, PhD and postdoctoral traineeship, each institute of the Institut Pasteur International Network (RIIP) contributes to the transmission of knowledge with the training of young researchers all around the world. In this context, doctoral and postdoctoral programmes, study and traineeship fellowships are available to scientists. Alongside training, dynamism and attractiveness of RIIP will result in the creation of 4-year group for the young researchers.
Scientists at the Institut Pasteur have identified a series of mutations in a gene that are associated with 4% of cases of, otherwise unexplained, severe male infertility. This discovery offers new opportunities not only in terms of an early diagnosis of male infertility associated with these mutations but also enables clinicians to provide counseling regarding the freezing of sperm or eggs to the couples concerned about procreation. Male infertility is a known risk factor for developing certain forms of cancer and this study reinforces the need for a long-term medical follow-up of patients carrying these mutations.
Paris, september 30, 2010
Worldwide, one couple in seven is affected by infertility, and male factors can account for 30 to 50% of these cases. Although many genes are known to be essential for human germ cell development, there are surprisingly few single gene changes that have been conclusively demonstrated to cause a failure of sperm production in humans. Researchers at the Unit of Human Developmental Genetics at the Institut Pasteur directed by Ken McElreavey, have identified a gene whose mutations explain some severe cases of infertility in humans.
In 2009, the same team had already shown that mutations in the same gene, called NR5A1, could be the cause of ovarian insufficiency in women1. These studies led them to investigate the contribution of mutations in this gene in men with otherwise unexplained infertility.
The study, led by Dr. Bashamboo, investigated 315 men affected by unexplained infertility. The sequencing of the gene NR5A1 for these individuals revealed seven mutations (4%) associated with severe spermatogenic failure. Similar mutations were not observed in more than 2000 control samples. This may be the most frequent single gene defect that is associated with male infertility known to date.
In vitro functional studies demonstrated a marked reduction in the biological activity of the mutant proteins as compared to the normal protein. The mutant proteins failed to adequately induce the expression of downstream target genes of NR5A1, that are known to be involved in the development of the testis and biosynthesis of certain testicular hormones.
This reasearch has a direct medical impact. Some men carrying mutations show a progessive reduction in sperm quality and quantity over time. For the carriers of potentially pathogenic mutations in the gene it would be advisable to consider having childen early in their reproductive lives or they may consider freezing their sperm.
Furthermore, some studies show that male infertility may be a risk factor for developing prostate or testicular cancer. In addition, mice that lack the Nr5a1 gene also develop other diseases (hypertension, obesity etc.). It is therefore necessary to perform long term follow-up studies in men carrying NR5A1 mutations to assess their risk of developing other diseases.
This work was carried out in collaboration with AP-HP, Hôpital Tenon (Paris) and the Biological Resource Center GERMETHEQUE, which provided samples for the study. This was a collaborative study with a team from the University College London.
(1)See our press communication of 26 February 2009
Human Male Infertility Associated with Mutations in NR5A1 Encoding Steroidogenic Factor 1, The American Journal of Human Genetics, en ligne le 30 septembre 2010.
Anu Bashamboo (1), Bruno Ferraz-de-Souza (2), Diana Lourenço (1), Lin Lin (2), Neil J. Sebire (3),
Debbie Montjean (1), Joelle Bignon-Topalovic (1), Jacqueline Mandelbaum (4), Jean-Pierre Siffroi (5),
Sophie Christin-Maitre (6), Uppala Radhakrishna (7), Hassan Rouba (8), Celia Ravel (1,4), Jacob Seeler (9), John C. Achermann (2) and Ken McElreavey (1)
(1) Human Developmental Genetics, Institut Pasteur, 75724 Paris, France
(2) Developmental Endocrinology Research Group, Clinical and Molecular Genetics Unit, UCL Institute of Child Health, London WC1N 1EH, UK
(3) Department of Paediatric Histopathology, Great Ormond Street Hospital for Children, London WC1N 3JH, UK
(4) UPMC, APHP, Hôpital Tenon Service d’Histologie et de Biologie de la Reproduction, Paris 75020, France
(5) APHP-ER9 UPMC Service de Génétique et d’Embryologie Médicales, Hôpital Armand Trousseau, Paris 75012, France
(6) Service d’Endocrinologie, Hôpital Saint-Antoine, Paris 75012, France
(7) The Cancer Center, Creighton University, Omaha, NE 68178, USA
(8) Human Genetics Unit, Institut Pasteur of Morocco, Casablanca 20100, Morocco
(9) Nuclear Organisation and Oncogenesis Unit, INSERM U579, Institut Pasteur, Paris 75724, France