Revisiting the mechanism of common fragile sites instability

Michelle DEBATISSE

Directrice de l'Unité Dynamique de l'information génétique : bases fondamentales et cancer Institut Curie Séminaire Départemental Génomes et Génétique

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - jeudi 24 mai 2012 11:00 Contact: Guy-Franck Richard (guy-franck.richard@pasteur.fr)

"How do dopamine neurons compute reward prediction error signals ?"

Pr Naoshige Ushida, PhD

Associate Professor, Center for Brain Science, Dpt of Molecular & Cellular Biology, Harvard University, Cambridge, MA, USA

Salle Jules Bordet, rdc du bâtiment Metchnikoff - jeudi 24 mai 2012 12:00 Contact: Pierre-Marie Lledo et Mariana Alonso (mariana.alonso@pasteur.fr)

Structural Mapping of the Protein Universe; One Cell at a Time

Dorit HANEIN

Sanford Burnham Medical Research Institute, La Jolla, USA SEMINAIRE DU DEPARTEMENT DE VIROLOGIE

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - jeudi 24 mai 2012 12:30 Contact: Félix Rey (rey@pasteur.fr)

De la création des écoles vétérinaires pour combattre la peste bovine, à son éradication : 1761 - 2011

Jean-Claude Manuguerra

Institut Pasteur - Paris

Salle Jules Bordet, rdc du bâtiment Metchnikoff - jeudi 24 mai 2012 14:00 Contact: Annick OPINEL et Michel GARENNE (annick.opinel@pasteur.fr ; michel.garenne@pasteur.fr)

The molecular interplay between bacteriophages and hosts : A matter of divirusity

Rob LAVIGNE

Division of Gene Technology, Katholieke Universiteit Leuven, Belgium Séminaire organisé par le département de Microbiologie Abstract : Through evolution, bacterial viruses have co-evolved with their host, resulting in a close molecular interaction during the bacteriophage infection cycle. After ejection of phage DNA into the host bacterium, intracellular phage development entails a number of tightly programmed steps, which finally result in lysis of the host, induced by phage-encoded cell wall hydrolases. The transition to a phage-oriented bacterial metabolism early in infection is mediated by proteins that either inhibit or co-opt critical bacterial metabolic processes to ensure proper viral development. For this, phages encode a wealth of mostly unknown proteins. We currently investigate these molecular hijacking and lysis processes in Pseudomonas infecting phages, using the "-omics" approaches to better understand the fundamental biology of this interplay. From an applied perspective, we aim to "harvest" novel enzymes and relevant proteins for medical, biotechnological and industrial applications.

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - vendredi 25 mai 2012 11:30 Contact: Laurent Debarbieux (laurent.debarbieux@pasteur.fr)

Steroid hormone signalling and hemocyte activation in Drosophila

Antonio JACINTO

Instituto de Medicina Molecular, Portugal

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - vendredi 25 mai 2012 12:00 Contact: Jean-Pierre LEVRAUD (jean-pierre.levraud@pasteur.fr)

Pharmacological silencing of HIV transcription

Susana Valente

The Scripps Research Institute, Florida USA SEMINAIRE DU DEPARTEMENT DE VIROLOGIE

Salle 014-015, rdc du bâtiment Lwoff - mercredi 30 mai 2012 12:00 Contact: Olivier Schwartz (schwartz@pasteur.fr)

Critical role for LFA1 in T cell exhaustion and protection against a chronic viral infection

Antonio PEIXOTO

Department of Molecular Mechanisms of Mycobacterial Infections - UMR 5089 CNRS/UPS Institut de Pharmacologie et de Biologie Structurale – Toulouse SEMINAIRE DU DEPARTEMENT GENOMES ET GENETIQUE

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - jeudi 31 mai 2012 11:00 Contact: Ludovic Tailleux (ludovic.tailleux@pasteur.fr)

New ABC transporters in Leishmania: involvement in the infectivity and drug resistance

Francisco Gamarro Conde

Instituto de Parasitologia y Biomedicina "Lopez-Neyra" CSIC Grenade - Espagne

Salle 014-015, rdc du bâtiment Lwoff - jeudi 31 mai 2012 12:00 Contact: Gérald Spaeth (gerald.spaeth@pasteur.fr)

" Lipids Turn On Protein Kinases "

Dr Thomas LEONARD

Department of Structural and Computational Biology, Max F. Perutz Laboratories, Vienna ( ** Séminaire du Département de Biologie Structurale et Chimie / DBSC ** )

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - jeudi 31 mai 2012 12:00 Contact: Ludovic Sauguet - Pierre Maisonneuve (ludovic.sauguet@pasteur.fr - pierre.maisonneuve@pasteur.fr)

Salmonella secretion systems related to colonization and infection of poultry

Wolfgang KÖSTER

Vaccine and Infectious Disease Organization – International Vaccine Center, University of Saskatchewan, Canada Séminaire organisé par le Département de Microbiologie Abstract Salmonella enterica subspecies enterica serovar Enteritidis (Salmonella Enteritidis) is a leading causative agent of gastroenteritis in humans. This pathogen also colonizes the intestinal tract of poultry and can spread systemically in chickens. Transfer to humans usually occurs through undercooked or improperly handled poultry meat or eggs. Salmonella Enteritidis employs two Type III Secretion systems (T3SS) which deliver so-called effector proteins directly into host cells. T3SS-1, T3SS-2 are located on Salmonella Pathogenicity Islands, SPI-1 and SPI-2. The bacterial twin-arginine transport (Tat) pathway is responsible for the translocation of folded proteins across the cytoplasmic membrane. In order to study the roles of T3SS-1, T3SS-2 and the Tat system in infection and colonization of Salmonella Enteritidis in chickens, we constructed chromosomal deletion mutantions in genes, which are essential components of these systems. When tested in chickens, the Tat system inactivation did not substantially affect cecal colonization, but it delayed systemic infection. Similar observations were made testing SPI-1 and SPI-2 mutants. Altogether, our data demonstrated that all three systems might play a role in Salmonella Enteriditis pathogenesis.

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - jeudi 31 mai 2012 14:00 Contact: Elie Dassa (elie.dassa@pasteur.fr)

How to orchestrate assembly of prokaryotic molybdoenzymes

Axel MAGALON

Laboratoire de Chimie Bactérienne (UMR 7283) CNRS - Institut de Microbiologie de la Méditerranée - Aix-Marseille Université Séminaire organisé par le département de Microbiologie Abstract Transition metals play a critical role in the functioning of numerous proteins and enzymes. In order for metals to play their essential roles, they must be acquired, assembled into proper cofactors, and appropriately incorporated into their target enzymes, all while avoiding the toxicity associated with free metals; these are complicated processes typically requiring a large number of gene products. While some of the gene products involved in metalloenzyme maturation are part of the general house-keeping machinery of a cell, others have functions unique to the maturation of a single metalloenzyme. One of the most prominent example is molybdenum, a transition metal found associated to a pterin-based cofactor being as such part of the active site of a wide variety of enzymes present in all domains of life. The importance of those molybdenum pterin cofactor-containing enzymes is exemplified by their ubiquity, their roles in metabolic diversity and global geochemical cycles. Based on recent studies from our group as well as others, significant progresses have been made toward understanding the assembly of folded and active prokaryotic molybdoenzymes. Expression of prokaryotic molybdoenzymes is controlled by transport of Mo or W (often replacing Mo in the cofactor) into the cell, cofactor biosynthesis, transcriptional regulation of apo-protein expression and riboswitch mechanisms. The biosynthesis of prokaryotic molybdoenzymes is an intricate process, requiring the synthesis of various subunits in the cytoplasm, incorporation of the metal and pterin cofactors, subunit assembly, correct subcellular targeting/transport and in many cases anchoring of the complex to the inner membrane. A series of devoted molybdoenzyme maturation proteins are crucial in various stages of enzyme maturation. Interaction with a specific lipid constitutes, in some cases, the final biogenesis step and is required for activity of the membrane-bound molybdoenzyme. Finally, compartmentalization of respiratory processes in “respirazones” could be achieved through a tightly controlled subcellular localization of individual respiratory complexes. During this talk, I will highlight the progress on prokaryotic molybdoenzyme assembly which has been done through the concerted and joined efforts from researchers in biology, chemistry and physics.

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - vendredi 1 juin 2012 11:30 Contact: Cécile Wandersman (cecile.wandersman@pasteur.fr)

The Drosophila intestinal epithelium : from immunity to physiology

Bruno LEMAITRE

UPLEM, École Polytechnique Fédérale de Lausanne (EPFL), Swiss

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - vendredi 1 juin 2012 12:00 Contact: Benoit ROBERT (benoit.robert@pasteur.fr)

Regulation of actin and plasma membrane dynamics by BAR domain proteins

Prof. Pekka LAPPALAINEN

Institute of Biotechnology P.O. Box 56 00014 University of Helsinki Finland Séminaire du Département de Biologie Cellulaire et Infection

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - lundi 4 juin 2012 12:00 Contact: Nathalie Sauvonnet (nathalie.sauvonnet@pasteur.fr)

Novel functions of sphingosine-1-phosphate in neuroinflammation

Tomasz KORDULA

Virginia Commonwealth University School of Medicine, Etats-Unis.

Salle Jules Bordet, rdc du bâtiment Metchnikoff - lundi 4 juin 2012 12:00 Contact: Sandra Pellegrini (sandra.pellegrini@pasteur.fr)

" Towards understanding the two-membrane spanning type 2 secretion system "

Prof. Wim HOL

Professor of Biochemistry and Biological Structure Head, Biomolecular Structure Center University of Washington - Seattle

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - mercredi 6 juin 2012 12:00 Contact: Rémi FRONZES & Olivera FRANCETIC (remi.fronzes@pasteur.fr - olivera.francetic@pasteur.fr)

Gene regulation in three dimensions

Wouter DE LAAT

Hubrecht Institute, Utrecht The Netherlands SEMINAIRE DU DEPARTEMENT GENOMES ET GENETIQUE

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - jeudi 7 juin 2012 11:00 Contact: Emmanuelle Fabre et Jost Enninga (emmanuelle.fabre@pasteur.fr jost.enninga@pasteur.fr)

" Structural Biology in Drug Design of Tropical Parasitic Diseases "

Prof. Wim HOL

Professor of Biochemistry and Biological Structure Head, Biomolecular Structure Center University of Washington - Seattle

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - jeudi 7 juin 2012 11:30 Contact: Rémi FRONZES - Geneviève MILON (remi.fronzes@pasteur.fr - genevieve.milon@pasteur.fr)

Eradication et décision en santé publique : quels critères prendre en compte ? exemple de la rage

Florence RIBADEAU DUMAS

Institut Pasteur - Paris

Salle Jules Bordet, rdc du bâtiment Metchnikoff - jeudi 7 juin 2012 14:00 Contact: Annick OPINEL et Michel GARENNE (annick.opinel@pasteur.fr ; michel.garenne@pasteur.fr)

Two-Partner Secretion in Bordetella : the FHA/FhaC model

Centre d'Infection et d'Immunité de Lille - CNRS UMR 8204 - INSERM U1019 – Institut Pasteur de Lille - Université Lille-Nord de France Séminaire organisé par le Département de Microbiologie Abstract Two-Partner Secretion (TPS) systems are widespread among Gram-negative bacteria. They are dedicated to the secretion of ‘TpsA’ proteins that fold as long beta helices and carry out various functions in their host organisms. These proteins cross the plasma membrane in a Sec-dependent fashion and then are translocated across the outer membrane by a specific transporter of the Omp85/TpsB superfamily. In the whooping cough agent Bordetella pertussis, the FHA adhesin is secreted by the transporter FhaC, a member of the Omp85/TpsB superfamily. Our goal is to decipher the molecular principles that govern two-partner secretion using the FHA/FhaC model system. A conserved domain in FHA has been identified that is likely to initiate the vectorial folding of the protein at the cell surface. We have shown that the chaperone /protease DegP is involved in FHA secretion in B. pertussis. FhaC forms a 16-stranded beta barrel in the outer membrane that is obstructed in the form that was crystallized. We have obtained evidence for an important conformational change that opens the channel.

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - vendredi 8 juin 2012 11:30 Contact: Olivera Francetic (olivera.francetic@pasteur.fr)

Immunity and Immunopathology in experimental Malaria

Jean LANGHORNE

MRC National Institute for Medical Research, London, Grande-Bretagne

Salle Jules Bordet, rdc du bâtiment Metchnikoff - vendredi 8 juin 2012 12:00 Contact: Paulo Vieira (paulo.vieira@pasteur.fr)

Tissue refinement - from disorder to order

Buzz BAUM

Cancer Research UK Senior Research Fellow - MRC - Laboratory of Molecular Cell Biology, United Kingdom

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - vendredi 8 juin 2012 12:00 Contact: François SCHWEISGUTH (francois.schweisguth@pasteur.fr)

Regulatory B10 cells in inflammation, autoimmunity and cancer

Thomas TEDDER

Duke university School of Medicine, Durham, Etats-Unis

Salle Jules Bordet, rdc du bâtiment Metchnikoff - vendredi 8 juin 2012 16:00 Contact: Alexander Tang (alexander.tang@pasteur.fr)

Neural stem and progenitor cells and the evolution of the cerebral cortex

Pr. Wieland B. HUTTNER

Max-Planck-Institute of Molecular Cell Biology and Genetics Dresden, Germany Séminaire du Département de Biologie Cellulaire et Infection

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - lundi 11 juin 2012 12:00 Contact: Arnaud Echard (arnaud.echard@pasteur.fr)

" Imaging Biological Function in 3D Across Scales: From Macromolecules to Cells to Tissues and Microbial communities "

Manfred AUER

Lawrence Berkeley National Laboratory, Berkeley ( ** Séminaire du Département de Biologie Structurale et Chimie / DBSC ** )

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - mercredi 13 juin 2012 12:00 Contact: Michael NILGES (michael.nilges@pasteur.fr)

Fundamental and applied aspects of lambda Red homologous recombination

Francis STEWART

Genomics, Technische Universitaet Dresden Séminaire du Département Génomes et Génétique

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - jeudi 14 juin 2012 11:00 Contact: Didier Mazel (didier.mazel@pasteur.fr)

" Molecular recognition at protein surfaces "

Ernest GIRALT

Institute for Research in Biomedicine, Parc Cientific de Barcelona ( ** Séminaire du Département de Biologie Structurale et Chimie / DBSC ** )

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - jeudi 14 juin 2012 12:00 Contact: Muriel DELEPIERRE (muriel.delepierre@pasteur.fr)

Role of basophils in protective immunity against parasitic infection and in allergy.

Hajime KARASUYAMA

Tokyo Medical and Dental university, Tokyo, Japon

Salle Jules Bordet, rdc du bâtiment Metchnikoff - jeudi 14 juin 2012 13:00 Contact: Pierre Bruhns (pierre.bruhns@pasteur.fr)

Les femmes pasteuriennes : de la « cuisine » à la paillasse

Sandra Legout

bibliothécaire- responsable du fonds patrimonial- Médiathèque Pasteur Dés 1889, les femmes sont présentes dans les laboratoires pasteuriens. D’abord « petites mains dans les cuisines » puis préparatrices, assistantes, elles vont lentement s’imposer dans l’univers de la recherche scientifique pasteurienne jusqu’à diriger des laboratoires. Rencontre avec les premières femmes scientifiques pasteuriennes …

Musée de l'Institut Pasteur - jeudi 14 juin 2012 15:00 Contact: Mueil Hilaire

Regulation of cell division by Polo and Aurora family kinases

Pr. Francis BARR

EP Abraham Professor of Mechanistic Cell Biology Department of Biochemistry, University of Oxford Séminaire du Département de Biologie Cellulaire et Infection

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - lundi 18 juin 2012 12:00 Contact: Arnaud Echard et Robert Weil (arnaud.echard@pasteur.fr, robert.weil@pasteur.fr)

Lynn Margulis and the endosymbiotic origin of eukaryotes: a historical reevaluation

Antonio Lazcano

Facultad de Ciencias, UNAM, Mexico

Salle Jean-Paul Aubert, rdc du bâtiment Fernbach - lundi 18 juin 2012 14:00 Contact: Miria Ricchetti (mricch@pasteur.fr)

Chimiothèques locale, nationale, européenne, à propos de la chimiothèque du CERMN et de ses contes

Prof. Sylvain RAULT

Centre d'Etudes et de Recherche sur le Médicament de Normandie, Université de Caen Basse-Normandie ( ** Séminaire du Département de Biologie Structurale et Chimie / DBSC ** )

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - mardi 19 juin 2012 12:00 Contact: Thérèse MALLIAVIN (therese.malliavin@pasteur.fr)

Controlling the indeterminate : prospectives on Hepatitis B and childhood vaccination in West and Central Africa

Tamara GILES-VERNICK

Institut Pasteur - Paris

Salle Jules Bordet, rdc du bâtiment Metchnikoff - jeudi 21 juin 2012 14:00 Contact: Annick OPINEL et Michel GARENNE (annick.opinel@pasteur.fr ; michel.garenne@pasteur.fr)

" " Characterisation of the FhuA-pb5 complex: interaction between an E. coli outer-membrane transporter and the Receptor Binding Protein of phage T5 "

Cécile BREYTON

Institut de Biologie Structurale, UMR 5075 CNRS/CEA/UJF, Grenoble ( ** Séminaire du Département de Biologie Structurale et Chimie / DBSC ** )

Salle 014-015, rdc du bâtiment Lwoff - jeudi 28 juin 2012 14:00 Contact: Nadia IZADI-PRUNEYRE (<mailto:nadia.izadi@pasteur.fr>nadia.izadi@pasteur.fr)

Chromatin dynamics and function

Department of Cell Biology and Infection - Mini-Symposium

Institut Pasteur Department of Cell Biology and Infection Mini-symposium Auditorium du Centre d’Information Scientifique 14:00-14:45: Dirk Schübeler (Friedrich Miescher Institute, Basel, Switzerland) DNA sequence determinants of gene repression 14:45-15:10: Romain Koszul (Institut Pasteur, Spatial Regulation of Genomes) Capturing and modeling yeast chromosome organization in wild type and structural mutants 15:10-15:35: Christian Muchardt (Institut Pasteur, Epigenetic Regulation) Human nuclear Argonaute couples chromatin-mediated silencing to regulation of alternative splicing 15:35-16:00: Oliver Bischof (Institut Pasteur, Nuclear Organisation and Oncogenesis) The long and the short reach of noncoding RNAs in cellular senescence 16:00-16:30: Coffee break 16:30-16:55: Sarra Merzouk (Institut Pasteur, Mouse Molecular Genetics) Gene expression and chromatin folding at the X-inactivation center during imprinted X-chromosome inactivation 16:55-17:20: Hélène Bierne (Institut Pasteur, Bacteria-Cell Interactions) An epigenetic regulator targeted by a bacterial pathogen: the dark side of the BAHD1 chromatin-silencing complex 17:20-17:45: Laurence Arbibe (Institut Pasteur, Molecular Microbial Pathogenesis) The interception of MAPK nuclear signaling by Shigella: impact on chromatin information during transcription of immune genes 17:45-18:30: Geneviève Almouzni (Institut Curie, Nuclear dynamics and genome plasticity) Variation on chromatin assembly from the nucleosome to higher order organization 18:30-19:00: Cocktail

Salle de Conférence du CIS (auditorium) - samedi 30 juin 2012 14:00 Contact: Anne Dejean et Christophe Zimmer (lambrech@pasteur.fr)

Small RNA-directed control of the plant and animal innate immune responses

Lionel NAVARRO, C.R. CNRS

Institut de Biologie, ENS, Paris, France Séminaire du Département de Biologie Cellulaire et Infection

Amphithéâtre Jacques Monod, sous-sol bâtiment Jacques Monod - lundi 29 octobre 2012 12:00 Contact: Philippe Sansonetti (philippe.sansonetti@pasteur.fr)